- 124 on Queen Hotel & Spa
- 627 on King Bed and Breakfast
- Abacot Hall Bed & Breakfast
- Antique Slumber Old Town Bed and Breakfast
- Apple Tree Historic B&B
- Best Western Colonel Butler Inn
- Blue Skies Bed & Breakfast
- Canterbury Inn
- Explorer House Bed & Breakfast
- Gatekeeper's Retreat
- Graystone Bed & Breakfast
- Green Oaks B&B
- Hilton Garden Inn Niagara-on-the-Lake
- Historic Lyons House
- Historic Wilson-Guy House
- In Elegance
- Luisa's Suite Retreat
- Oban Inn & Spa Niagara-on-the-Lake
- Orchid Inn
- Queen Regent Bed & Breakfast
- Residence & Conference Centre - Niagara-On-The-Lake
- Riverbend Inn and Vineyard
- School House Bed and Breakfast C.1859
- Serendipity Bed and Breakfast
- South Landing Inn
- Via Veneto - Old Town
- Vineyard Villa
- WeatherPine Inn
- White Oaks Conference & Resort Spa
- Williams Gate B&B Private Suites
- Woodbourne Inn